Underground reservoirs in Žlutý kopec

Starobrno Brewery (in Czech: Pivovar Starobrno) is a Czech brewery located in the city of Brno. It was built as a successor of the brewery founded in 1325, as a part of Cistercian convent. The brewery was named Starobrno Brewery only in the second half of the 19th century. In 2009, Starobrno Brewery produced more than one million hectoliters of beer. The same year, the brewery merged with the Royal Brewery of Krušovice and became a part of the Dutch brewing company Heineken N.V.

St Thomas's Abbey (or the Königskloster) (Czech: Starobrněnský klášter) is an Augustinian abbey and church located in Brno in the Czech Republic. The geneticist and abbot Gregor Mendel was its most famous religious leader to date, who between 1856 and 1863 conducted his experiments on pea plants in the monastery garden. His experiments brought forth two generalizations which later became known as Mendel's Laws of Inheritance. The Abbey is unique amongst modern Augustinian foundations because it is not called a priory, and indeed it has an abbot (Prälat - prelate) whereas all other existing Augustinian friaries are led by a prior.

Mendel Museum (Czech: Mendelovo muzeum) has been an institution of Masaryk University in Brno, Czech Republic, since 2007. The museum was established in 2002 with the international co-operation of a number of organizations. The principal role in the creation of the museum itself was played by the Austrian society VFG and affiliated scientists and patrons. The museum is located within the precincts of the Augustinian abbey in Old Brno, where the abbot and scientist Gregor Johann Mendel lived and worked.

Špilberk Castle (Czech: hrad Špilberk; German: Spielberg, locally Špilas) is a castle on the hilltop in Brno, Southern Moravia. Its construction began as early as the first half of the 13th century by the Přemyslid kings and completed by King Ottokar II of Bohemia. From a major royal castle established around the mid-13th century, and the seat of the Moravian margraves in the mid-14th century, it was gradually turned into a huge baroque citadel considered the harshest prison in the Austrian Empire, and then into barracks. This prison had always been part of the Špilberk fortress and is frequently referenced by the main character, Fabrizio, in Stendhal's novel, The Charterhouse of Parma.

The Diocese of Brno (Latin: Dioecesis Brunensis) is a Latin diocese located in the city of Brno in the ecclesiastical province of Olomouc in the Czech Republic.

The Deutsche Technische Hochschule Brünn was a institute of technology in Brno (German: Brünn). It existed from 1849 to 1945 and instruction was in German. At the time, Brno was a multicultural city with both Czech and German populations. A technical university serving both nationalities was proposed, but fell through, resulting in the establishment of the Brno University of Technology in 1899. From 1860, the Deutsche Technische Hochschule was located on Komenského Square in Brno, and in 1910 a second building was built beside the first one. During the Nazi occupation, it was planned for the university to be relocated to Linz, but this did not happen. In 1945, the Deutsche Technische Hochschule Brünn was closed and its property transferred to the Brno University of Technology.

The Red Church (Czech: Červený kostel) is a Protestant Church in Brno, Czech Republic. It was designed by architect Heinrich von Ferstel and built up between 1863 and 1867. Nowadays it is used by the Evangelical Church of Czech Brethren. The building is a cultural monument.

The University of Defence (in Czech: Univerzita obrany, UO) is the only military institution of higher education of the Czech Armed Forces. Established as of 1 September 2004, it was formed by merging three existing institutions: Military Academy Brno (established in 1951), the Military University of the Ground Forces Vyškov (established in 1947) and the Military Medical Academy Hradec Králové (re-established in 1988). Unlike public universities, as a state institution with limited authority the University of Defence is the only university-type school in the Czech Republic that is not a legal entity but an element in the Ministry of Defence structure. Thus, the rights of the Minister of Education towards the public universities are performed by the Minister of Defence. University of Defence is responsible for education of military professionals and experts engaged in national security system, defence industry and public administration. The available Bachelor’s, Master’s and doctoral degree programmes, both in full- and part-time mode, focus on military and national security fields. University of Defence represents the Army of the Czech Republic’s defence and security research and development centre. The fields of science fostered at the University of Defence primarily relate to defence applied research, to forces and population protection, or the economics or medicine fields applied to military. It is the only Czech institute where subjects such as weapons and ammunition, fighting vehicles, radars, population protection, fire support control, field surgery, radiobiology or toxicology are taught.

The Orel ("eagle" in Czech) is a Moravia-based Czech youth movement and gymnastics organization which emerged between 1896–1909 as Catholic Church-supported competitor of another Czech sport movement Sokol (founded 1862), oriented more nationalistic and rather anti-Catholic. Orel movement, defining itself as "Christian sport organization", still exists and currently has about 19,000 members. Since 1921 is member of international sport association FICEP. Party always cooperated in close with Moravian-Silesian Christian Social Party and later with the Czechoslovak People's Party. A similar organization with the same name was organized also in the Slovene Lands and in Croatia, where it successfully competed with the local Sokol movement. It was dissolved in the early 1930s by the following dictatorship in the Kingdom of Yugoslavia.

The Moravian Gallery in Brno (Czech: Moravská galerie v Brně) is the second largest art museum in the Czech Republic, established in 1961 by merging of two older institutions. It is situated in five buildings: Pražák Palace, Governor's Palace, Museum of Applied Arts, Jurkovič House and Josef Hoffmann Museum. Since 1963 the gallery has organized the International Biennial of Graphic Design Brno (Czech: Mezinárodní bienále grafického designu Brno).

Moravian Library (Czech: Moravská zemská knihovna) is the second largest library in the Czech Republic. Located in Brno, Czech Republic, it is a universal research library and the regional library of the South Moravian Region.

The Constitutional Court of the Czech Republic (Czech: Ústavní soud České republiky) is the supreme constitutional court in the Czech Republic and the de facto highest and most powerful court in the land. The Constitution provides that the Court be "charged with protection of constitutional rule" and as such its primary jurisdiction is to review and rule on questions of constitutionality. In this respect, it is similar in functionality to the Supreme Court of the United States, but is distinct from the Supreme Court of the Czech Republic. Of all the various levels of the Czech Judiciary it is the one created with the greatest specificity in the Constitution. The decisions of the Court are final, cannot be overturned and are considered a source of law, similar to precedents in a common law system.Although the Court itself was established only in 1993, its Czechoslovak predecessor was already provided for in the Constitution of 1920, making Czechoslovakia the first country in the world with a system of judicial review by a specialized court (although the court itself first convened after the Austrian one). It was later adopted by many other countries.