Špilberk Castle

The Orel ("eagle" in Czech) is a Moravia-based Czech youth movement and gymnastics organization which emerged between 1896–1909 as Catholic Church-supported competitor of another Czech sport movement Sokol (founded 1862), oriented more nationalistic and rather anti-Catholic. Orel movement, defining itself as "Christian sport organization", still exists and currently has about 19,000 members. Since 1921 is member of international sport association FICEP. Party always cooperated in close with Moravian-Silesian Christian Social Party and later with the Czechoslovak People's Party. A similar organization with the same name was organized also in the Slovene Lands and in Croatia, where it successfully competed with the local Sokol movement. It was dissolved in the early 1930s by the following dictatorship in the Kingdom of Yugoslavia.

The Red Church (Czech: Červený kostel) is a Protestant Church in Brno, Czech Republic. It was designed by architect Heinrich von Ferstel and built up between 1863 and 1867. Nowadays it is used by the Evangelical Church of Czech Brethren. The building is a cultural monument.

The Moravian Gallery in Brno (Czech: Moravská galerie v Brně) is the second largest art museum in the Czech Republic, established in 1961 by merging of two older institutions. It is situated in five buildings: Pražák Palace, Governor's Palace, Museum of Applied Arts, Jurkovič House and Josef Hoffmann Museum. Since 1963 the gallery has organized the International Biennial of Graphic Design Brno (Czech: Mezinárodní bienále grafického designu Brno).

The Diocese of Brno (Latin: Dioecesis Brunensis) is a Latin diocese located in the city of Brno in the ecclesiastical province of Olomouc in the Czech Republic.

Mendel Museum (Czech: Mendelovo muzeum) has been an institution of Masaryk University in Brno, Czech Republic, since 2007. The museum was established in 2002 with the international co-operation of a number of organizations. The principal role in the creation of the museum itself was played by the Austrian society VFG and affiliated scientists and patrons. The museum is located within the precincts of the Augustinian abbey in Old Brno, where the abbot and scientist Gregor Johann Mendel lived and worked.

The Constitutional Court of the Czech Republic (Czech: Ústavní soud České republiky) is the supreme constitutional court in the Czech Republic and the de facto highest and most powerful court in the land. The Constitution provides that the Court be "charged with protection of constitutional rule" and as such its primary jurisdiction is to review and rule on questions of constitutionality. In this respect, it is similar in functionality to the Supreme Court of the United States, but is distinct from the Supreme Court of the Czech Republic. Of all the various levels of the Czech Judiciary it is the one created with the greatest specificity in the Constitution. The decisions of the Court are final, cannot be overturned and are considered a source of law, similar to precedents in a common law system.Although the Court itself was established only in 1993, its Czechoslovak predecessor was already provided for in the Constitution of 1920, making Czechoslovakia the first country in the world with a system of judicial review by a specialized court (although the court itself first convened after the Austrian one). It was later adopted by many other countries.

The Hotel International in Brno, is one of the most important and best preserved buildings in the Brussels style in the Czech Republic. The eleven-story hotel was built between 1959 - 1962 designed by Ernest Krejza and Milos Kramoliše. The building is inspired by the style of Brazilian architect Oscar Niemeyer, There are also features of late functionalism. The hotel was opened on 29 June 1962. From its inception to 24 December 2013, the building was a cultural monument. The hotel was operated from 1962 to 1989 by the state company Inter Brno,. At the time of opening of the hotel had 301 rooms with a capacity of 697 people. It had about 300 employees. After the Velvet Revolution in 1989, the hotel was privatized and ownership passed to Hotel International Brno, Inc., founded in 1992.

St Thomas's Abbey (or the Königskloster) (Czech: Starobrněnský klášter) is an Augustinian abbey and church located in Brno in the Czech Republic. The geneticist and abbot Gregor Mendel was its most famous religious leader to date, who between 1856 and 1863 conducted his experiments on pea plants in the monastery garden. His experiments brought forth two generalizations which later became known as Mendel's Laws of Inheritance. The Abbey is unique amongst modern Augustinian foundations because it is not called a priory, and indeed it has an abbot (Prälat - prelate) whereas all other existing Augustinian friaries are led by a prior.

Masaryk University (MU) (Czech: Masarykova univerzita; Latin: Universitas Masarykiana Brunensis) is the second largest university in the Czech Republic, a member of the Compostela Group and the Utrecht Network. Founded in 1919 in Brno as the second Czech university (after Charles University established in 1348 and Palacký University existent in 1573–1860), it now consists of ten faculties and 35,115 students. It is named after Tomáš Garrigue Masaryk, the first president of an independent Czechoslovakia as well as the leader of the movement for a second Czech university.In 1960 the university was renamed Jan Evangelista Purkyně University after Jan Evangelista Purkyně, a Czech biologist. In 1990, following the Velvet Revolution it regained its original name. Since 1922, over 171,000 students have graduated from the university.

Authors' Reading Month is the largest Central European literary festival running annually since 2000. The organiser is the Větrné mlýny Publishers, (Brno, CZ) and the festival takes place every July 1–31. Every day 2–3 readings take place performed by writers of the host country and writers from the festival guest country. At present (as of 2016), the festival takes place in 5 cities simultaneously, spanning 4 countries: Brno and Ostrava (CZ), Wroclaw (PL), Košice (SK), and Lviv (UA). In 2020 the festival guest country was Hungary.

Starobrno Brewery (in Czech: Pivovar Starobrno) is a Czech brewery located in the city of Brno. It was built as a successor of the brewery founded in 1325, as a part of Cistercian convent. The brewery was named Starobrno Brewery only in the second half of the 19th century. In 2009, Starobrno Brewery produced more than one million hectoliters of beer. The same year, the brewery merged with the Royal Brewery of Krušovice and became a part of the Dutch brewing company Heineken N.V.

The Underground reservoirs in Žlutý kopec are located between Tvrdého and Tomešova street on Žlutý kopec. The first of the historic reservoirs was built in 1872 to improve the quantity and quality of Brno's water supply. The reservoir was supplemented with another similar one at the end of the 19th century, and two decades later by a third reservoir consisting of two concrete tanks. The reservoirs remained in operation until 1997, when they were disconnected from the water supply network.In autumn 2019, an entrance was built to allow access to the reservoirs, but has so far only been used for operational purposes. In the same year they were declared a cultural monument. In July 2020 the first underground reservoir was open to the public.