Penfield Central School District

The Daisy Flour Mill is a restaurant and assembly hall located in what was once a flour mill on Irondequoit Creek. It is located on Blossom Road in the town of Penfield, New York, just across the creek from Ellison Park. Originally built in 1840, it is the last remaining mill on Irondequoit Creek.The weatherboard building was added to the National Register of Historic Places on June 26, 1972.

Horace and Grace Bush House, also known as Bush-Fellows Residence, is a historic home located at Penfield in Monroe County, New York. It is an 1821 Federal style structure with Greek Revival period additions and embellishments. The main block is a two-story, five bay post and beam structure sheathed in clapboard. It is regular and symmetrical in massing and plan. The house was moved to its current location in 1931.It was listed on the National Register of Historic Places in 1994.

Hipp–Kennedy House is a historic home located at Penfield in Monroe County, New York. The main body of the house was built in 1838 and is in the Greek Revival style. The frame building is composed of a two-story, three-bay main block with center entrance flanked by identical 1+1⁄2-story wings. The north wing of the residence is believed to incorporate the remnants of a log dwelling built about 1804.It was designated as a Penfield landmark in 1982 and was listed on the National Register of Historic Places in 1994.

The Charles Finney School is a private, non-denominational Christian school offering a college preparatory program to students from Pre-Kindergarten through Grade 12. The school has developed a reputation for both student outreach and as a strong competitor, despite its small size. Finney is accredited by both the Middle States Association of Colleges and Schools and the Association of Christian Schools International and is the only school in the Greater Rochester, New York region to have achieved both. Finney students come from 24 different school districts in the region, as well as from nine different countries through the school's International Student Program.

Penfield High School (PHS) is a public high school in Penfield, New York, United States. It offers a comprehensive curriculum for students in grades 9–12.

WFKL (93.3 MHz) is an FM radio station licensed to Fairport, New York, and serving the Rochester metropolitan area. The station, branded as "Fickle 93.3", broadcasts an adult hits radio format, or what the station refers to as "We Play Everything," similar to Jack FM. It is and owned by Stephens Media Group which also owns WRMM and WZNE. The studios are in the First Federal Plaza building in downtown Rochester. WFKL has an effective radiated power (ERP) of 4,400 watts. The transmitter is off Five Mile Line Road in Penfield, New York.

Dayton's Corners School is a one-room school building in the town of Penfield, New York. Erected in 1857, it sits at the corner of Plank Road and Creek Street and was part of Penfield District #9. It is the only one-room school house in the area that is still standing and has not been converted to some other use.The building was part of the Webster Central School District between 1948 and 1991, although it was only used as a schoolhouse until the early 1960s. The building has been maintained by Penfield's recreation department since 1976; it was purchased by the town in 1991. The original school grounds are adjacent to the school building. The school and its grounds are used by fourth-grade teachers in the Penfield Central School District and other local school districts as part of the state-mandated local history curriculum.The red brick building was designated a Penfield Landmark in 1983 and added to the National Register of Historic Places on July 5, 2001. A coloring book called "A Day at Dayton's Corners" was created in 2010 as part of the town of Penfield's bicentennial celebrations.

Samuel Rich House is a historic home located at Penfield in Monroe County, New York. It was originally built in 1816 as a 1+1⁄2-story, gable-roofed frame dwelling in the rural vernacular building tradition. It was substantially enlarged in 1832 with the addition of an elegant 2-story, five-bay, Federal style, hipped roof, main block. Also on the property are three contributing structures: a chicken coop, brick smokehouse, and the stone foundation of a frame barn.It was listed on the National Register of Historic Places in 1987.

The Mud House is a historic home located at Penfield in Monroe County, New York. It is a 1+1⁄2-story, 24-by-38-foot (7.3 by 11.6 m) rectangular earthen building with gable roof. It was constructed about 1836 of clay, puddled with straw, and then rammed into forms above a fieldstone foundation and is a rare surviving example of rammed-earth construction and was part of the Brown Brothers Nursery owned by Don Brown. It was listed on the National Register of Historic Places in 1978.

Irondequoit Creek is a stream in eastern Monroe County, New York that feeds Irondequoit Bay. It begins in rural West Bloomfield in Ontario County, flowing north into the town of Mendon in Monroe County. Accumulating a few small tributaries, it twists eastward back into the Ontario County town of Victor, then back north into Monroe County, where it flows through the towns of Perinton and Penfield on its way to the bay. It also skirts the edge of the combined town and village of East Rochester. The creek is believed to lie in a valley carved out by a pre-glacial Genesee River, which at the time would have flowed into Lake Ontario where Irondequoit Bay does today. Glacial debris caused the river to be rerouted as the glaciers retreated, leaving only the comparatively small creek (Rogers 1893). The first settlements in the town of Penfield sprang up along the creek, as its waters were well suited to mills. The Daisy Flour Mill, previously a restaurant, was the last of over a dozen mills that once used the creek's waters. The creek's valley caused a problem for the engineers of the original Erie Canal, who ultimately had to build the Great Embankment aqueduct to bridge the valley near Bushnell's Basin (Fairchild 1896:134, Farley 2010, Schoff:505). The creek is stocked annually with fish by the New York State Department of Environmental Conservation for year-round fishing.

The Officer Daryl R. Pierson Memorial Post Office Building is a historic post office building located at East Rochester in Monroe County, New York. It was designed and built 1936–1937, and is one of a number of post offices in New York State designed by the Office of the Supervising Architect of the Treasury Department, Louis A. Simon. The one story brick building is in the Colonial Revival style and has a roof crowned by a flat topped cupola. The entrance features fluted Doric columns and a blind fanlight with sculpted eagle. The interior features a mural titled "Recreation Hours" by Bernard GussowIt was listed on the National Register of Historic Places in 1988. In 2015, the post office was named for Daryl Pierson, an East Rochester native and Rochester police officer who was killed in the line of duty the previous year; the building was officially dedicated on June 4, 2016.

St. John Fisher University is a private university in Pittsford, New York. It is named after John Fisher, an English Catholic cardinal and saint. It was named St. John Fisher College until July 1, 2022.