The Anglesey Central Railway (Welsh: Lein Amlwch, Amlwch Line) was a 17.5-mile (28.2 km) standard-gauge railway in Anglesey, Wales, connecting the port of Amlwch and the county town of Llangefni with the North Wales Coast Line at Gaerwen. Built as an independent railway, the railway opened in portions from 1864 to 1867. Due to financial troubles the railway was sold to the London and North Western Railway in 1876, which invested significantly in the infrastructure. Operation continued under various companies during the 20th century, but passenger services were withdrawn in 1964 as part of the Beeching Axe. Industrial freight services continued until 1993. The railway's tracks remain and local groups have demonstrated an interest in restoring services as a heritage railway. As of 2023, the middle 7 miles (11 km) from Llangefni station via Llangwyllog to Llannerch y Medd station have largely been cleared of vegetation, with a view to reopening.
The sustainable transport charity Sustrans has proposed to use the route as a cycle path (rail trail). The Welsh Assembly Government, in partnership with Network Rail, commissioned a feasibility study into the reopening of the line, which started in early 2011. However, as of February 2020 nothing had come of the study.