Museum of Antiquities in Vilnius

Vilnius University Library or VU Library (also VUL) is the oldest and one of the largest academic libraries of Lithuania. It was founded in 1570 by the Jesuits and as such is nine years older than Vilnius University. VU Library holds 5.4 million documents on shelves measuring 166 kilometres (103 mi) in length. The holdings, accessible to members of the university and wider public, include some of the oldest manuscripts, incunabula and engravings in Lithuania and Eastern Europe. At present the library has 36 thousand users. Vilnius University Library consists of the Central Library which is situated near the Presidential Palace, the Scholarly Communication and Information Centre in Saulėtekis and libraries of faculties and centres that are scattered all around the city.

The Church of St. Johns, St. John the Baptist and St. John the Apostle and Evangelist (Lithuanian: Vilniaus Šv. Jono Krikštytojo ir Šv. Jono apaštalo ir evangelisto bažnyčia) is located at the Old Town of Vilnius, Lithuania and dominates the university (Vilnius University) ensemble. Since the times of the Jesuit Academy professors and students used to pray here, and Vilnius theologians gave sermons. It was a place for performances and disputes, where theses were defended and kings greeted.

European Humanities University (Belarusian: Еўрапейскі гуманітарны ўніверсітэт (ЕГУ), Lithuanian: Europos humanitarinis universitetas (EHU), Russian: Европейский гуманитарный университет (ЕГУ)) is a private, non-profit liberal arts university founded in Minsk, Belarus, in 1992. Following its forced closure by the Belarusian authorities in 2004, EHU relocated to Vilnius (Lithuania) and thus continues its operations as a private university. EHU offers high-residence and low-residence undergraduate, graduate and postgraduate degree programs in the field of humanities and social sciences. The university has been headquartered in Vilnius, Lithuania, since authorities expelled it from Belarus in 2004. The university intends to return to Minsk.From 1992 to 2004 EHU was a non-state establishment of undergraduate and post-graduate education in Belarus. In 2004, due to government opposition, EHU was forced to terminate its activities in Belarus. However, thanks to political, administrative, and financial support from the European Union, the Nordic Council of Ministers, the Governments of Lithuania, other European countries, and the United States, NGOs and foundations like MacArthur Foundation, Carnegie Corporation of New York, and others, EHU resumed its operation in Vilnius, Lithuania and opened bachelor's and master's degree programs for Belarusian students in autumn 2005. In March 2006 the Government of Lithuania granted EHU the official status of a Lithuanian university. After the mass protests of the Belarusian presidential election of 2010, many EHU students and teachers were imprisoned by the KDB. The university said it would work with students to help them in their education despite the circumstances.In academic year 2020/21 EHU serves around 680 mostly Belarusian students, offering undergraduate and graduate degree programs and promoting research in the humanities and social sciences. About two-thirds of EHU's students attend via online programs and reside in Belarus. About one-third attend courses on campus in Vilnius. Teaching languages – Belarusian, Russian, some courses are taught in English, German and French. EHU ranks second among private universities in Lithuania.

Vilnius University (Lithuanian: Vilniaus universitetas) is a public research university, which is the first and largest university in Lithuania, as well as one of the oldest and most prominent higher education institutions in Central and Eastern Europe. Today, it is Lithuania's leading research institution, ranked among the Top 29% Higher Education Institutions in the world.The university was founded in 1579 as the Jesuit Academy (College) of Vilnius by Stephen Báthory. It was the third oldest university (after the Cracow Academy and the Albertina) in the Polish–Lithuanian Commonwealth. Due to the failure of the November Uprising (1830–1831), the university was closed down and suspended its operation until 1919. In the aftermath of World War I, the university saw failed attempts to restart it by the local Poles, Lithuanians, and by invading Soviet forces. It finally resumed operations as Polish Stefan Batory University in August 1919. After the Soviet invasion of Poland in September 1939, the university was briefly administered by the Lithuanian authorities (from October 1939), and then after Soviet annexation of Lithuania (June 1940), punctuated by a period of German occupation after Operation Barbarossa, from 1941 to 1944, when it was administrated as the Vilnius State University. In 1945, the Polish community of students and scholars of Stefan Batory University was transferred to Nicolaus Copernicus University in Toruń. After Lithuania regained its independence in 1990, following the dissolution of the Soviet Union, it resumed its status as one of the prominent universities in Lithuania. The university has facilities in Kaunas and Šiauliai. It has 15 academic faculties that offer more than 200 study programs for over 24,000 students. Non-academic departments of the university include the Cultural Centre, Health and Sports Centre, Library, Museum, Botanical Gardens and other institutions. Since 2016, Vilnius University has been a member of a network of prestigious universities–the Coimbra Group–and since 2019, it has belonged to the European University Alliance (ARQU).The Vilnius University Foundation was established on 6 April 2016, becoming the first university endowment in Lithuania. Underground beneath the old university's building is the cenotaph of 11 Old Prussian tribes along with the small temple of four gods and four goddesses from Baltic mythology.

Kardynalia Palace is a no-longer-existing palace in Vilnius, located at the corner of Pilies and St. John's Streets, right next to the university Church of St. Johns. The palace was constructed between the 16th and 17th centuries for the Radziwiłł family. One of its owners at the time was Cardinal and Bishop of Vilnius Jerzy Radziwiłł, from whom the palace derived its name. The palace remained in the possession of the Radziwiłłs for about 300 years, and in the 19th century, it became a government building. Damaged in 1944, it was not rebuilt after World War II, and in 1956, the palace's remnants were demolished to make way for residential buildings.

Pac Palace is a building in the Old Town of Vilnius, Lithuania, on Šv. Jonų street, close to Vilnius University. Currently it houses the Polish embassy.

Vilnius University Astronomical Observatory is an astronomical observatory of Vilnius University. It was founded in 1753 by initiative of Thomas Zebrowski. The observatory is the fourth oldest observatory in the Europe. While the observatory is no longer able to make astronomical observations due to light pollution in Vilnius (observations are carried out at Molėtai Astronomical Observatory), it continues scientific research.

Łopacińskis (Sulistrowskis) Palace (Lithuanian: Lopacinskių (Sulistrovskių) rūmai, Polish: Pałac Łopacińskich (Sulistrowskich)) is a building in Vilnius Old Town, Skapo st (Skapo st. 4). Currently it is owned by the State Language Inspectorate and Vilnius Archdiocese Economy Council.

The House of the Signatories (Lithuanian: Signatarų namai, previously known as Sztral's House) is a Lithuanian historic landmark on Pilies Street, Vilnius, where on February 16, 1918, the Act of Independence of Lithuania was signed by twenty members of the Council of Lithuania.

The Presidential Palace (Lithuanian: Prezidentūra), located in Vilnius Old Town, is the official office of the president of Lithuania. The palace dates back to the 14th century and during its history it has undergone various reconstructions, supervised by prominent architects, including Laurynas Gucevičius and Vasily Stasov. In 1997 the palace became the official seat of the president of Lithuania.

Daukantas Square in Vilnius, Lithuania is located in the Old Town, in front of the Presidential Palace. Its name commemorates a progenitor of the 19th-century Lithuanian national revival, Simonas Daukantas. In the late 19th century it had a monument of Mikhail Nikolayevich Muravyov-Vilensky. The square hosts state ceremonies. It has also been the site of demonstrations and rallies.

The de Choiseul (de Reuss) Palace (Lithuanian: Šuazelių (de Reusų) rūmai; Polish: pałac de Choiseulów (de Reussów)) is a building in Simonas Daukantas square, Vilnius Old Town, Lithuania. Currently it is used as dwellings and "Copy1" company subsidiary.