Schomburg Center for Research in Black Culture

The 135th Street station is a station on the IRT Lenox Avenue Line of the New York City Subway. Located at the intersection of 135th Street and Lenox Avenue in Harlem, it is served by the 2 and 3 trains at all times. The 135th Street station was constructed for the Interborough Rapid Transit Company (IRT) as part of the city's first subway line, which was approved in 1900. Construction on the tunnel segment that includes the 135th Street station started on October 2 of the same year. The station opened on November 23, 1904. The station platforms were lengthened in 1910. The 135th Street station contains two side platforms and three tracks. The station was built with tile and mosaic decorations. The platforms contain exits to Lenox Avenue's intersection with 135th Street and are not connected to each other within fare control. The platforms contain elevators from the street, which make the station compliant with the Americans with Disabilities Act of 1990.

The Dark Tower was a townhouse on 108 West 136th Street in Harlem.

The Harlem YMCA is located at 180 West 135th Street between Lenox Avenue and Adam Clayton Powell Jr. Boulevard in the Harlem neighborhood of Manhattan, New York City. Built in 1931-32, the red-brown brick building with neo-Georgian details was designed by the Architectural Bureau of the National Council of the YMCA, with James C. Mackenzie Jr. as the architect in charge. It replaced the building from 1919 across the street. Inside the building is a mural by Aaron Douglas titled "Evolution of Negro Dance." The building was declared a National Historic Landmark in 1976, and was designated a New York City Landmark in 1998.

Harlem Hospital Center, branded as NYC Health + Hospitals/Harlem, is a 272-bed, public teaching hospital affiliated with Columbia University. It is located at 506 Lenox Avenue in Harlem, Manhattan, New York City and was founded in 1887.The hospital was established to provide healthcare to the citizens of the neighborhood. Initially, the hospital served as a holding area for patients to be transferred to Randalls and Wards Islands and Bellevue Hospital. With the wave of the African Americans who moved to New York after World War I, the hospital soon outgrew its initial building. After acquiring land, a new building opened on April 13, 1907. The hospital developed a teaching program that is affiliated with Columbia University, and has continued to serve the Harlem neighborhood since its inception.

The Lincoln Theatre is a theater located on 135th Street near Lenox Avenue in Harlem, New York City. It opened in 1915 and was the first theater in a then predominantly white neighborhood in Harlem to cater to black audiences. The theater reached its peak of fame in the 1920s, when entertainers such as Bessie Smith, Florence Mills, and Fats Waller headlined. (Fats Waller had been hired as the organ player of the theater when he was fifteen years old.) The Lincoln Theatre was the only place in New York where Ma Rainey performed.

The Mother African Methodist Episcopal Zion Church, also known as "Mother Zion", located at 140–148 West 137th Street between Adam Clayton Powell Jr. Boulevard and Lenox Avenue in the Harlem neighborhood of Manhattan, New York City, is the oldest African-American church in New York City, and the "mother church" of the African Methodist Episcopal Zion conference.The church was designated a New York City Landmark on July 13, 1993.

The James Weldon Johnson Residence is a historic apartment house located at 187 West 135th Street, Harlem, Manhattan, New York City, New York. It is here where James Weldon Johnson (1871-1938) lived from 1925 until his death. In addition to being a composer, songwriter, and author, he was an outspoken advocate for civil rights, working in various roles at the NAACP, including as its General Secretary during his residency here. The building was declared a National Historic Landmark in 1976.

The Abyssinian Baptist Church is a Baptist megachurch located at 132 West 138th Street between Adam Clayton Powell Jr. Boulevard and Lenox Avenue in the Harlem neighborhood of Manhattan, New York City. It is affiliated with the National Baptist Convention, USA and American Baptist Churches USA. Its senior pastor is Johnnie D. Brooker, Jr.. The Abyssinian Baptist Church congregation traces its history to 1809, when seamen from the Ethiopian Empire (then known as Abyssinia) helped lead a protest against racially segregated church seating. Thomas Paul was an early minister. During the 20th century, prominent ministers of the church included Adam Clayton Powell Sr., Adam Clayton Powell Jr., and Samuel DeWitt Proctor. The church has served as an influential place for African-American spirituality, politics and community. The church worshiped in several places before building the present church structure Its present building was built in 1922–23 and was designed by Charles W. Bolton & Son in Gothic Revival and Tudor Revival styles – it has also been described as "Collegiate Gothic". It features stained glass windows and marble furnishings. The church and its associated community house were designated a New York City Landmark on July 13, 1993.

The Hoofers Club was an African-American entertainment establishment and dancers' club hangout in Harlem, New York, that ran from the early 1920s until the early 1940s. It was founded and managed by Lonnie Hicks (1882–1953), an Atlanta-born ragtime pianist.

The Renaissance Ballroom & Casino was an entertainment complex at 2341–2349 Adam Clayton Powell Jr. Boulevard (Seventh Avenue) in the Harlem neighborhood of Manhattan in New York City. When opened in 1921, it included a casino, ballroom, 900-seat theater, six retail stores, and a basketball arena. It spanned the entire eastern frontage of Adam Clayton Powell Jr. Boulevard between 137th and 138th Streets.

Harlem Hospital School of Nursing was a training school for African-American women, which was established at Harlem Hospital in Harlem, New York City in 1923. It was founded due to the lack of nursing schools in New York that accepted African American women. Until 1923, the Lincoln Hospital School for Nurses in The Bronx was the only school that allowed the enrollment of Black women. When Mayor Hylan sought reelection in 1921, the NAACP and other community organizations lobbied the mayor to improve healthcare access. Around the same time, Lurline Vassall of Brooklyn, was denied entry to the Bellevue Hospital School of Nursing because of her race. Lurline's father William Vassall launched a campaign to open a school for black nurses. In response, Hylan's administration supported the creation of the Harlem Hospital School of Nursing.The school opened on January 3, 1923, with a class of twenty black women. It was a two and a half year program.The Harlem Hospital School of Nursing closed in 1977.

The Church of St. Mark the Evangelist is a Roman Catholic parish church in the Roman Catholic Archdiocese of New York, located at West 138th Street, near Lenox Avenue in northern Harlem, Manhattan, New York City. The address is 59-61 West 138th Street and 195 East Lenox Avenue. The parish was established in 1907 and has been staffed by the Holy Ghost Fathers since 1912. The Rev. Charles J. Plunkett, pastor, had a brick church built in 1914 to designs by Nicholas Serracino of 1170 Broadway for $12,000.