St. Longin's Rotunda

The Research Institute of Brewing and Malting (RIBM) (Czech: Výzkumný ústav pivovarský a sladařský, a.s.) in Czech - was founded in 1887 and since 1994 It has been a joint-stock company. It is one of the oldest scientific research institutes in the Central European region.

The First Faculty of Medicine of Charles University (Czech: 1. lékařská fakulta Univerzity Karlovy) is one of five medical faculties of Charles University, Prague, Czech Republic. Founded in 1348 at the same time as the university itself, it is the oldest medical faculty in Central Europe and the 11th oldest medical institution in the world. Situated in the centre of Prague, the faculty provides education in all fields of general medicine. It is regularly ranked in the top 1% of medical faculties globally (See ranking below). It is one of the five medical faculties of Charles University, alongside the Second and Third Faculties, both in Prague, and faculties in Plzen and Hradec Kralove.It has two student associations. One for the Czech parallel (SMC) and one for the English parallel (LF1 MEDSOC). The student associations have their offices in Faust House and are responsible for helping new students get used to the university life as well as organise all the parties and activities, including Freshers Week every September. The faculty has recently established a surgical society, which envisions the nurture of aspiring surgeons from all years by providing research opportunities, away clerkships and workshops.

The Antonín Dvořák Museum in Prague is a museum dedicated to the Czech composer Antonín Dvořák (1841–1904). It is part of the Czech Museum of Music which in turn is part of the National Museum. Since 1932, the museum has been housed in a Baroque style building which was designed by the famous architect Kilian Ignaz Dientzenhofer at the beginning of the 18th century, even though the house itself has no particular link with the Dvořák. It is situated in the north part of the New Town, about 15 minutes' walk from the city center of Prague.The museum displays photographs, newspaper cuttings, programmes and personal objects relating to the composer, including his viola and his piano. The building houses a unique collection of his manuscripts and correspondence, thus providing an important centre for research into Antonín Dvořák. Concerts are held there regularly, as well as seminars, lectures, and exhibitions. The museum also organizes an annual ceremony on the eve of the day of his death (1 May) at his grave in the Vyšehrad cemetery just south of the New Town district. There is also a matinee celebration on his birthday at his birthplace in Nelahozeves. The museum also takes care of the village house of his son-in-law, Josef Suk. There are also commemorative centres connected with Dvořák at other locations: there is a permanent exhibition at his country estate in Vysoká near Příbram, one in Zlonice and a memorial hall in Sychroy Castle near Turnov.

The Alcron Hotel Prague is a historic hotel in Prague on Štěpánská, just off Wenceslas Square, the main plaza in the city center. The Hotel Alcron was constructed by its owner/architect Alois Krofta, a local businessman, and opened in 1932. Krofta named the hotel after himself, taking the first two letters of his first and last names and adding an "n" to match the Alcron, a boat from Greek mythology. Pre-WWII guests included Charlie Chaplin and Winston Churchill, while Joachim von Ribbentrop and Karl Hermann Frank stayed at the hotel during the German occupation of Czechoslovakia. In 1948, the Alcron was nationalized by the new communist government.Following the fall of communism, the hotel closed in 1990, and the Krofta family filed claims for restitution in 1992. A legal battle ensued between two of Krofta's ex-wives and one of their daughters. The three women were eventually each given 1/3 ownership, which they each sold in 1995 to an Austrian-based firm, Crown WSF Ltd. In 1996, the new owners contracted Radisson SAS Hotels to manage the establishment upon its reopening. It was completely rebuilt and modernized at great expense, with the historic public rooms restored to their original Art Deco grandeur. It reopened on 1 August 1998 as the Radisson SAS Hotel Prague. Crown secured the right to use the Alcron name two years later, and the hotel was renamed Radisson SAS Alcron Hotel. The hotel was again renovated in 2008 and renamed Radisson Blu Alcron Hotel in 2009, when the Radisson SAS partnership ended. After twenty years of management by Radisson, the Alcron Hotel became independent and was renamed Alcron Hotel Prague on 1 January 2019. The Alcron hotel is home to the La Rotonde restaurant and the BeBop Bar.

The Drama Club (Czech: Činoherní klub) is a theatre located in Prague. The Drama Club was founded by Ladislav Smoček and Jaroslav Vostrý. The opening performance of Piknik took place on 3 March 1965. The actors in the 1970s and 1980s included Petr Čepek, Pavel Landovský, Josef Somr, Jiří Kodet, Jirina Trebicka, Libuše Šafránková and Josef Abrhám. On 19 November 1989, two days after the Velvet revolution, the Civic Forum was founded there. The Drama Club was awarded Alfréd Radok Award in category Theatre of the Year in 2002, and 2008. The current actors include Jaromír Dulava, Ivana Chýlková, Ondřej Vetchý and Petr Nárožný.

The Psychiatric Clinic in Prague is the oldest psychiatric hospital in the Czech Republic. Part of Prague's General Faculty Hospital, the clinic has five inpatient wards and also provides integrated day treatment. An expansion in 2000 added a day care centre for adolescents. The clinic's hot line, established in 1964, was the first of its kind in Europe.

Cambridge Business School s.r.o. is an educational institution providing professional management education in the BBA, MBA and LLM programmes. It was founded in 2010 and its headquarters are in Prague 1, Czech Republic.

Osvobozené divadlo (1926–1938) (Liberated Theatre or Prague Free Theatre) was a Prague avant-garde theatre scene founded as the theatre section of an association of Czech avant-garde artists Devětsil (Butterbur) in 1926. The theatre's beginnings were strongly influenced by Dadaism and Futurism, later by Poetism (a specific Czech art movement). The theatre was very leftist oriented, but it could also be critical of the Communists. One of the founders, Jiří Frejka, came up with the name in 1926. In the theatre both authorial plays and works by well-established modern authors; such as G. Apollinaire, A. Jarry, J. Cocteau, A. Breton, F. T. Marinetti, and V. Nezval were performed. The modern conception of the scene also laid more emphasis on lighting and the theatrical conception adjured more cooperation and contacts between actors and audience.

The New Town Hall (Czech: Novoměstská radnice, German: Neustädter Rathaus}) is the administrative centre of Prague's (medieval) New Town Quarter, or "Nové Město". In 1419 it was the site of the first of the three defenestrations of Prague.

St. Ignatius Church (Czech: Kostel svatého Ignáce) is a church in Prague, Czech Republic. Located on Charles Square, the church was designed by Carlo Lurago in the early Baroque style, and built between 1655 and 1677. The church was built as part of the new Nove Mesto residence of the city's Jesuits, the third largest Jesuit complex in Europe, and dedicated to their patron saint and founder of the Jesuit Order, St. Ignatius of Loyola. The top of the facade carries a statue of St. Ignatius Loyola, placed there in 1671, with a halo surrounding his whole body. This feature was considered controversial at the time it was installed, as such a decoration was only considered appropriate for statues of Jesus Christ. The decorative painting of the exterior was carried out by Jan Jiří Heinsch and the sculpture work was that of Matěj Václav Jäckel. The interior is characterised by stucco decoration and features statues of several Jesuit and Czech saints. Many of the interior furnishings of the church date from around 1770, added by Jesuit painter Ignác Raab, who lived briefly at the complex.

Charles Square (Czech: Karlovo náměstí) is a city square in the New Town of Prague, Czech Republic. At roughly 80,550 m² it is one of the largest squares in the world and was the largest town square of the medieval Europe. Founded in 1348 as the main square of the New Town by Charles IV, it was known as Dobytčí trh (Cattle Market) from the 15th century and finally named after its founder in 1848. The central portion of the square was turned into a park in the 1860s. The square is now one of the main transport hubs of the city centre with Karlovo náměstí metro station and numerous tram lines and busy roads crossing it in all directions.

Lucerna Palace (Czech: Palác Lucerna) is an entertainment and shopping complex in the New Town quarter of Prague, Czechia. In 2017, it was named a national cultural monument.