City of Alachua Downtown Historic District

Alachua ( ə-LATCH-oo-ay) is the second-largest city in Alachua County, Florida and the third-largest in North Central Florida. According to the 2020 census, the city's population was 10,574. Alachua has one of the largest bio and life sciences sectors in Florida and is the site for the Santa Fe College Perry Center for Emerging Technologies.

Newnansville, Florida was one of the first American settlements in the interior of Florida. It became the second county seat of Alachua County in 1828, and one of the central locations for activity during the Second Seminole War, during which time it was one of the largest cities in the State. In the 1850s, the Florida Railroad bypassed Newnansville, resulting in the county seat being moved to the new town of Gainesville in 1854. Consequently, Newnansville began to decline, and when a second railway bypassed the town in 1884, most of its residents relocated and formed the new City of Alachua. By 1900, Newnansville was deserted. The site is approximately 1.5 miles northeast of Alachua, on S.R. 235 off of US 441. Containing partial walls of two cemeteries, the town site was added to the National Register of Historic Places on December 4, 1974.

Santa Fe High School is a high school serving grades 9–12 from the Alachua-High Springs area in the northwestern part of Alachua County, Florida. It is located in Alachua, Florida and a part of the Alachua County Public Schools.

Fracture is an Internet-based photo finishing service. Fracture's main service is printing photos directly onto glass. The company is based in Gainesville, Florida.

The Battle of San Felasco Hammock took place during the Second Seminole War, fought by Florida's Seminole Indians to prevent their removal to the Arkansas Territory in accordance with the Indian Removal Act of 1830. Euro-American settlers established the town of Newnansville, Florida, around Fort Gilleland. The site upon which both Fort Gilleland and Newnansville once stood is now encompassed by the city of Alachua, Florida. The San Felasco Hammock is currently part of San Felasco Hammock Preserve State Park.

Warren's Cave (or Warren Cave) is a dry karst cave in Alachua County, Florida. It is the longest dry cave in Florida, with more than 4 miles (6.4 km) of mapped passages. The cave is located on the margin of the Cody Scarp near the San Felasco Hammock Preserve State Park, northwest of the city of Gainesville. The property on which the entrance to Warren's Cave is located, the Warren Cave Nature Preserve, is owned by the National Speleological Society. Warren's cave was probably formed by a high water table when sea levels in the Quaternary Period were elevated close to 100 feet (30 m) above current sea level, corresponding to the Wicomico terrace.By local tradition, Warren's cave was discovered by and named after Colonel John Warren, who led government troops at the Battle of San Felasco Hammock during the Second Seminole War. The cave was a popular local attraction in the late 19th century, mentioned in tourist guides. Wooden stairs were built leading to the entrance of the cave in a sinkhole in an attempt to make it a commercial attraction. Only some 400 feet (120 m) of passages were known until 1959. Since then, the discovery of several narrow passages ('squeezes') has greatly extended the known part of the cave.

Hague is an unincorporated community in Alachua County, Florida, United States. It is located on Alachua County Road 237 between U.S. Route 441 and the Deerhaven Subdivision line of the CSX Railroad, between the cities of Alachua and Gainesville.

San Felasco Hammock Preserve State Park is a Florida State Park in Alachua County, Florida. It is located northwest of Gainesville, Florida on CR 232 (Millhopper Road), just south of the town of Alachua.

Traxler is a ghost town located in Alachua County, Florida, just north of the city of Alachua.

Turkey Creek Preserve is a 375-acre preserve located in the Turkey Creek community of Alachua County, Florida.

Saint Francis Catholic Academy is a private, Roman Catholic high school in Gainesville, Florida. It is located in the Roman Catholic Diocese of St. Augustine.

Florida Lakewatch, established in 1986, is a volunteer water-monitoring program coordinated through the University of Florida. This project is headquartered at Gainesville, Florida, within the campus of the University of Florida. Florida Lakewatch has in its history obtained funding from a number of sources, but currently is mostly funded by the state of Florida.