Moraine Park Technical College

The University of Wisconsin–Oshkosh, Fond du Lac Campus (abbreviated as UWO Fond du Lac and formerly known as the University of Wisconsin–Fond du Lac) is a branch campus of the University of Wisconsin–Oshkosh and a member of the Universities of Wisconsin. It is located on 62 acres (25 ha) in Fond du Lac, Wisconsin. Prior to its merger with UW–Oshkosh in July 2018, the campus was a member of the University of Wisconsin Colleges. As of 2022, the campus enrolls 258 students, making it the third-smallest of the UW branch campuses. In October 2023, UW System President Jay Roth announced that the campus would cease in-person instruction following the Spring 2024 semester, citing enrollment declines and increasing costs.

Marian University is a private Roman Catholic university in Fond du Lac, Wisconsin. It was founded in 1936 by the Congregation of Sisters of Saint Agnes, which continues to sponsor the university today. Marian University has an enrollment of approximately 2,000 undergraduate and graduate students. Seventy-one percent of students are women. Ninety-four percent of students receive financial aid. Approximately 32% of undergraduate students live on campus.

Fond du Lac High School ("Fondy High") is a comprehensive public high school in Fond du Lac, Wisconsin administered by the Fond du Lac School District. Opened in 2001, the school replaced the former Goodrich High School, which was the city's high school from 1922 to 2001. Fond du Lac High School has an enrollment of approximately 2000 students. Fond du Lac High School's athletic teams are known as the Cardinals.

Winnebago Lutheran Academy, also known as WLA, is a private Lutheran high school in Fond du Lac, Wisconsin, associated with the Wisconsin Evangelical Lutheran Synod (WELS).

The William I. Cole House is located in Fond du Lac, Wisconsin.

The Rudolph and Louise Ebert House is located in Fond du Lac, Wisconsin.

The Fond du Lac Light is a lighthouse located at the entrance to the harbor and Yacht Club, in Fond du Lac, Wisconsin. It is located on the southern end of Lake Winnebago. It is currently incorporated in the Fond du Lac City Seal. Construction of the lighthouse started in May 1933 and was completed shortly after October. It replaced an ordinary red light that previously marked the harbor entrance. After years of neglect it was saved from demolition in 1967 and restored in 1993 with help from local community organizations. The lighthouse is also the focal point of the seasonal holiday lights in Lakeside Park; a cutout of Rudolph the Red-Nosed Reindeer is adorned to the light house using the red light on top of the lighthouse as the nose. Pending major rebuild for being crooked for the past 25 years.

The Fond du Lac County Fair is an annual county fair that takes place every July in Fond du Lac, Wisconsin, USA. The first Fond du Lac County Fair took place on September 29, 1852 in Rosendale. The Fond du Lac County Fairs' motto is "Education, Entertainment and Affordable Family Fun." Musical acts headlining the 2015 Grandstand include Grand Funk Railroad, (July 16, 2015) Great White & Slaughter, (July 17, 2015) Montgomery Gentry (July 18, 2015) and other family entertainment such as the Fondy Fair 5K Run/Walk on July 18, 2015. Other Grandstand shows include a tractor pull, horse pull, truck pull, BMX stunt show and a demolition derby. The only cancelled years were: 1917–18 & 1942–45. In 2020, officials had to limit attendance and cancel concerts & rides caused by the COVID-19 pandemic. The fairgrounds was used as an automobile racing facility in the early 20th century. Past winners include Bill Endicott in 1912.

The George and Mary Agnes Dana House is located in Fond du Lac, Wisconsin.

Aetna Station No. 5 is located in Fond du Lac, Wisconsin.

St. Mary's Springs Academy (SMSA, formerly St. Mary's Springs High School) is a Catholic, private, coeducational system serving grades 3-year-old preschool through high school in Fond du Lac, Wisconsin, associated with the Archdiocese of Milwaukee. It was founded by the Sisters of Saint Agnes in 1909.

The First Baptist Church of Fond du Lac is a church located in Fond du Lac, Wisconsin. It was added to the National Register of Historic Places in 1986 for its architectural significance.