Houston County Farm Center

The Alabama College of Osteopathic Medicine (ACOM) is a private medical school in Dothan, Alabama. It is the first osteopathic medical school in the state and is believed to be the first osteopathic medical school in the nation established by a regional not-for-profit hospital.It is accredited by the American Osteopathic Association's Commission on Osteopathic College Accreditation (COCA). Graduates of the college receive a Doctor of Osteopathic Medicine degree (D.O.). The first class graduated in 2017.

Dothan Preparatory Academy is located in Dothan, Alabama, USA. It is located on U.S. Highway 231 inside Ross Clark Circle, about 1.5 miles (2.4 km) north of the southern tip of Ross Clark Circle. The high school district roughly runs down U.S. Highway 84, which runs east to west through the heart of Dothan.

Rip Hewes Stadium is a 10,000-seat stadium located in Dothan, Alabama. It is primarily used as the home of the Dothan High School football and soccer teams, as well as other sporting and community events. It formerly shared home field with Dothan High School and Northview High School. Evangelist Billy Graham once held an evangelistic campaign event here during his second trip through Dothan. His previous event was held at the First Baptist Church, Dothan. Due to the overwhelming crowds at the First Baptist Church, his evangelistic campaign was held at Rip Hewes Stadium on his second trip to the area. Although it was pouring rain, crowds poured into the stadium to hear the gospel message.

The Wiregrass Museum of Art (formerly the Dothan Municipal Light and Water Plant) is an art museum in Dothan, Alabama. The museum was founded in 1988 and is located in the city's former power and water plant. The building was listed on the Alabama Register of Landmarks and Heritage in 1990 and the National Register of Historic Places in 1991.

The Main Street Commercial District is a historic district in Dothan, Alabama. The district covers 18 acres (7 ha) and portions of 6 blocks in Dothan's historic commercial district. At the time of the nomination, it contained 68 contributing properties, however many have been demolished in the intervening years. Dothan was founded on the site of Poplar Head Springs, which was an important crossroads in the Muscogee lands. Logging was the area's first major industry, but did not develop until after the Civil War. Settlers began building saw mills and blacksmith shops, and encouraged commercial development by donating land and bricks to new settlers. The town was incorporated in 1885, and began a period of great expansion with the coming of the Alabama Midland Railway in 1889. The oldest buildings in the district date from the 1900s, including the three-story Wadlington Hotel (built 1900, demolished 2012) and the Neoclassical First National Bank (1907). Several brick warehouses were also constructed along the railroad line. A symbol of the town's rapid growth, a five-story office building was completed in 1914. Cotton had replaced lumber as the economic engine of the region, but the boll weevil outbreak of the 1910s sent Dothan into a recession. Fewer buildings date from beyond this period, including the Malone Motor Company Building (1923) and the Kress Building (1928). The importance of the downtown core declined after the 1960s, as retail establishments relocated to outlying areas.The district was listed on the National Register of Historic Places in 1983.

The Dothan Opera House is a historic performing arts venue in Dothan, Alabama. It was built in 1914–15 as a municipal auditorium by the growing town. Seating 800, it opened October 8, 1915, with a performance by a local orchestra. The building was designed by Atlanta architectural firm Morris & Morris in a blend of Classical Revival and Italianate styles. The first floor features three central arched entry doors, with a pair of arched windows on either side. The second story is dominated by Ionic pilasters, four in the center section, and three on each side. The pilasters support a limestone architrave beneath a denticulated cornice and parapet roof. On the parapet, in line with each column in the center section is a figurine of an opera singer. A mural painted on the backdrop of the stage depicts the building under construction and some of the more famous acts to have performed in the theater.A new civic center was built across the street in 1971, and the opera house was refurbished at the same time. The building was listed on the National Register of Historic Places in 1977.

Dothan Civic Center is a 3,100-seat multi-purpose arena located in Dothan, Alabama. It hosts local sporting events and concerts. Ultimate Fighting Championship 12 was held at Dothan Civic Center. Recently (November. 10, 2018), actor and comedian Rodney Carrington performed.

The Federal Building and U.S. Courthouse, also known as U.S. Post Office and Court House, is a historic government building in Dothan, Alabama.

Howell School is a historic school building in Dothan, Alabama. The school was built in 1902 to replace the previous grammar school that had burned in 1901. It was named in 1916 for the recently deceased Robert Graves Howell, who was mayor when the first grammar school was built. A second school was built in 1911, and Howell was replaced by Minnie T. Heard Elementary School in 1942. After being used by the Salvation Army during World War II, the building was converted for use as a textile mill in 1947. A number of companies operated out of the building until 1952, when the Dothan Manufacturing Company took over the property. A one-story addition was constructed on the front of the building in 1965, obscuring the façade. The plant closed in 1997, and was sold to the Southern Alabama Regional Council on the Aging in 2003, before the city re-acquired it in 2008. The building combines Romanesque Revival and Renaissance Revival styles. The front façade originally had a mansard roofed tower, which was later removed. The outer three bays as well as the center bay project slightly, and are separated by pilasters with Corinthian terra cotta capitals. The entablature contains a plain architrave and frieze separated by a terra cotta band, and a pressed metal cornice with modillions. On the main façade, two dormers project from the hipped roof, while gabled dormers with round vents pierce the roof on the ends. A wing projects from the center of the rear of the building. All second floor window openings, as well as the multi-story windows on the wing, are arched with terra cotta hoodmolds.The school was listed on the National Register of Historic Places in 2013.

George Washington Carver High School was a segregated public high school in Dothan, Alabama serving African-American children from 1940 to 1968. In 1969 the students were integrated with white students at Dothan High School.

The Water Works Standpipe in Dothan, Alabama was listed on the National Register of Historic Places in 2016. It is a water tower built in 1897, when such were called standpipes. It is located on a triangular property now known as "Dixie Park", at the intersection of East Powell and North Saint Andrews St., .5 miles (0.80 km) north of Main St. in the Houston County portion of Dothan.The well driller was C.A. Ray, the builder of the standpipe was Guild & White, and the engineer was R. T. Ghent.

The Dothan station, also known as Atlantic Coastline Railroad Passenger Depot, is a historic train station in Dothan, Alabama. It was built in 1907 as the largest and busiest on the Atlantic Coast Line Railroad between Montgomery, Alabama, and Thomasville, Georgia and replaced a former freight depot. The Atlantic Coast Line merged with the Seaboard Air Line Railroad in 1967 to form the Seaboard Coast Line Railroad. In 1971, Amtrak took over passenger rail service in the United States and Dothan station was served by the Floridian until 1979. The depot is two stories, with a one-story portion on the western third. The first story's hipped roof wraps around the entire building, and has deep eaves supported by large brackets. All windows are two-over-two sashes with heavy granite sills and lintels. Double-leaf doors with transoms led to the two waiting rooms. The track side has a projecting bay that served as the ticketing window.The station was listed on the National Register of Historic Places in 1994.